Elevated Serum Protein Carbonyl and Reduced Antioxidant Capacity in Women with Preeclampsia

Abstract

Objectives: To determine the level of oxidative stress in preeclampsia by estimation of serum protein carbonyl content and antioxidants capacity in preeclampsia.

Methodology: This comparative cross-sectional study was conducted at dept. of Obs & District Teaching Hospital of D.I.Khan, KPK from March 2018 to January 2019. Forty clinically diagnosed preeclampsia women and 40 normal pregnant women aged between 27-35 years of the same gestational age were enrolled. The serum protein carbonyl content was determined through 2, 4-dinitrophenylhydrazone (DNPH) assay and serum antioxidant capacity was estimated through Ferric Reducing Ability of Plasma (FRAP) assay. Statistical analysis was performed through T- test/ T-statistics.

Results: Serum protein carbonyl content was significantly elevated (p<0.0001) in preeclamptic women as compared to normal pregnant women while serum antioxidant capacity (FRAP value) was significantly decreased (p<0.0001) in preeclampsia women as compared to the normal pregnant women.

Conclusion: Increased serum protein carbonyl content and reduced antioxidant capacity depict high levels of oxidative stress in women with preeclampsia that may play an important role in the initiation of endothelial dysfunction and expression of preeclampsia. Regular intake of adjuvant antioxidants supplementation can minimize the further progression of preeclampsia.